Can MTT be used to quantify the antioxidant activity of plant extracts?

نویسندگان

  • I A Muraina
  • M M Suleiman
  • J N Eloff
چکیده

Antioxidant properties of three different medicinal plant extracts from northern Nigeria were evaluated using the traditional qualitative 2,2, diphenyl-picrylhydrazyl (DPPH) method and a new 3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyltetrazolium bromide (MTT) microdilution method described here. The results were in agreement. It is a simple, rapid and inexpensive method compared with many other methods for determining quantitative antioxidant activity of plant extracts.

برای دانلود رایگان متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

New Usage of a Fluorometric Method to Assay Antioxidant Activity in Plant Extracts

      In the present study, an oxidative conversion of non-fluorescent dichlorofluoroscein (LDCF) to fluorescent dichlorofluoroscein (DCF) was used for detection of antioxidant properties of plant extracts. The rate of the reaction was followed by monitoring the formation of DCF as a function of time. The antioxidant assay was carried out for different concentrations of gallic acid, Salvia ...

متن کامل

Antioxidant, Antimicrobial Activity and Total Phenolic Content within the Aerial Parts of Artemisia absinthum, Artemisia santonicum and Saponaria officinalis

Three native Turkish medicinal and aromatic plants (Artemisia absinthum, Artemisia santonicum and Saponaria officinalis) were investigated to analyze their antioxidant activity, total phenolic content and antimicrobial activity. Their total antioxidant activity was determined by using a β-carotene bleaching assay and their antimicrobial activity was determined by utilizing an agar disc diffusio...

متن کامل

Total Phenol, Antioxidant and Antibacterial Activity of the Essential Oil and Extracts of Ferulago angulata ssp. angulata

 Background: In recent years, plants natural products have gained considerable attention as natural preservers for use in different industries. Due to their free radical scavenging activities, phenolic compounds can prevent a wide range of diseases such as cancers and cardiovascular and neurodegenerative diseases. For a long time, different Ferulago species have been in use as medicina...

متن کامل

Variability in antioxidant activity and phenolic profile in different parts of sunflower (Helianthus annuus L.) genotypes

Sunflower (Helianthus annuus L.) is largely consumed in Iran as an oleaginous and confectionery product. In this study, the total phenolic and flavonoid contents, antioxidant activity and phenolic composition in different parts of the seeds from six sunflower genotypes were investigated. The antioxidant activity of extract was evaluated by different assays. The total phenolic and flavonoid cont...

متن کامل

Anti-tumor Activity of Ferulago angulata Boiss. Extract in Gastric Cancer Cell Line via Induction of Apoptosis

AbstractFerulago angulata Boiss. known in Iran as Chavir, has some bioactive compounds having antioxidant activity. Because of its antioxidant activities, it sounded Chavir extract can be a good candidate for finding chemopreventive agents having inductive apoptosis properties on cancer cells. In this study, the cytotoxic effects and proapoptotic activities of Chavir’s leaf and flower extracts ...

متن کامل

ذخیره در منابع من

ذخیره در منابع من قبلا به منابع من ذحیره شده

{@ msg_add @}


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

عنوان ژورنال:
  • Phytomedicine : international journal of phytotherapy and phytopharmacology

دوره 16 6-7  شماره 

صفحات  -

تاریخ انتشار 2009